在文献“Fidelity of DNA polymerases in the detection of intraindividual variation of mitochondrial DNA”中提到, Taq扩增酶的错误率是在2x10-4--5之间，而Q5保真酶大概是10-6级别。具体可以看下这篇文献。
The fidelity of a DNA polymerase is usually expressed by the mean error rate per base per duplication (Keohavong and Thilly 1989). Taq-pol has an error rate between 2 × 10−4 (Saiki et al. 1988; Keohavong and Thilly 1989) and 2 × 10−5 (Eckert and Kunkel 1990; McInerney et al. 2014). Proofreading activity of the high-fidelity polymerases drops error rates to 10−6 or even lower (Cline 1996; Li et al. 2006; McInerney et al. 2014). Despite the higher error rate of Taq-pol compared to high-fidelity polymerases, the frequency of each error in the population of amplified molecules is still considered low (Lin et al. 2002). However, these errors may hinder the analysis of coamplification of different sequences (Pascual et al. 1994; Bracho et al. 1998), as in studies of heteroplasmy. |
