什么是Sanger测序中的Primer walking工作？

有人可以帮忙介绍下什么是Primer walking流程及工作原理嘛？

It is a DNA Sequencing Methods.
Pls refer textbooks or references for more details.
Primer walking is a step-by-step approach to sequencing long DNA templates from end to end that overcomes the inability of the Sanger chain termination method to read more than a few hundred bases in a single reaction. After an initial round of sequencing from a known sequence at one end of the template, each subsequent round is initiated from a new primer, which is based on the end of the sequence obtained from the previous reaction.

Keywords: primer walking; DNA sequencing; genome; gap closure; hexamer library

Ref: http://www.els.net/WileyCDA/ElsArticle/refId-a0005382.html

Primer Walking in the Finishing Lab at the Broad Institute; This reference give you an general idea of what it is.
Ref: http://www.lanl.gov/conferences/ ... %2520for%2520NM.pdf

参考小木虫上的答复。